Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides
K Misiura, I Durrant, MR Evans, MJ Gait - Nucleic acids research, 1990 - academic.oup.com
Non-nucleosidic phosphoramidite linker units suitable for use on commercial DNA synthesis
machines have been designed for the direct incorporation of biotin and a new reporter
group, phosphotyrosine, at multiple sites on synthetic oligonucleotides. The units are based
on a 3-carbon glyceryl backbone where the reporter group is attached to the 2-O-position
through a 3-aminopropyl spacer. 17-mer oligonucleotides were synthesized carrying at the
5′-end 1, 2, 4 or 8 biotinyl units or 1, 2, 4 or 8 phosphotyrosinyl units respectively and used …
machines have been designed for the direct incorporation of biotin and a new reporter
group, phosphotyrosine, at multiple sites on synthetic oligonucleotides. The units are based
on a 3-carbon glyceryl backbone where the reporter group is attached to the 2-O-position
through a 3-aminopropyl spacer. 17-mer oligonucleotides were synthesized carrying at the
5′-end 1, 2, 4 or 8 biotinyl units or 1, 2, 4 or 8 phosphotyrosinyl units respectively and used …
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